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insulin like growth factor 1  (R&D Systems)


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    R&D Systems insulin like growth factor 1
    Insulin Like Growth Factor 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+insulin+like+growth+factor+1/Recombinant+Human+IGF-I%2FIGF-1+Protein%2C+CF/bio_rxiv__64898__2026__01__13__699271-146-140-146
    Average 96 stars, based on 375 article reviews
    insulin like growth factor 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Transplantation of Human Embryonic Stem Cell-Derived Retinal Cells into the Subretinal Space of a Non-Human Primate
    Article Snippet: Briefly, embryoid bodies (EBs) were formed by treating undifferentiated hES colonies with dispase and type IV collagenase (Invitrogen, Grand Island, NY) and resuspended in approximately 150 100-cell clumps per milliliter in a six-well ultra-low attachment plate (VWR, Radnor, PA). .. These EBs were cultured for 3 days in the presence of mouse noggin (R&D Systems, Minneapolis, MN), human recombinant Dkk-1 (R&D Systems), and human recombinant insulin-like growth factor-1 (IGF-1; R&D Systems). .. On the fourth day, EBs were plated onto each poly-D-lysine-Matrigel (Collaborative Research, Inc., Bedford, MA) coated plates and cultured in the presence of DMEM/F12, B-27 supplement, N-2 Supplement (Invitrogen), mouse noggin, human recombinant Dkk-1, human recombinant IGF-1, and human recombinant basic fibroblast growth factor (bFGF; R&D Systems).

    Recombinant:

    Article Title: Transplantation of Human Embryonic Stem Cell-Derived Retinal Cells into the Subretinal Space of a Non-Human Primate
    Article Snippet: Briefly, embryoid bodies (EBs) were formed by treating undifferentiated hES colonies with dispase and type IV collagenase (Invitrogen, Grand Island, NY) and resuspended in approximately 150 100-cell clumps per milliliter in a six-well ultra-low attachment plate (VWR, Radnor, PA). .. These EBs were cultured for 3 days in the presence of mouse noggin (R&D Systems, Minneapolis, MN), human recombinant Dkk-1 (R&D Systems), and human recombinant insulin-like growth factor-1 (IGF-1; R&D Systems). .. On the fourth day, EBs were plated onto each poly-D-lysine-Matrigel (Collaborative Research, Inc., Bedford, MA) coated plates and cultured in the presence of DMEM/F12, B-27 supplement, N-2 Supplement (Invitrogen), mouse noggin, human recombinant Dkk-1, human recombinant IGF-1, and human recombinant basic fibroblast growth factor (bFGF; R&D Systems).

    Article Title: Construction and characterization of EGFP reporter plasmid harboring putative human RAX promoter for in vitro monitoring of retinal progenitor cells identity
    Article Snippet: .. So hESCs were transferred to low adherent dishes in order to form EBs in neural induction medium (NIM) containing 1 ng/ml noggin (R&D, 1976-NG), 3 μM IWR (R&D, 5439-DK/CF), and 5 ng/mL human recombinant insulin-like growth factor-1 (IGF-1) (R&D, 291-GI) in DMEM/F12 medium supplemented with 10% KSR, 0.1 mM nonessential amino acids, 2 mM L-glutamine, and 1% B27 (Gibco, 17,504–044) for 3 days. .. In the next step, the EBs were dissociated by Accutase (Millipore, SCR005) and were replated on 1 mg/ml laminin and 15 mg/ml Poly-L-ornithine (both from Sigma-Aldrich) coated 6-well tissue culture plates.

    Article Title: Lack of human relevance for rat developmental toxicity of flumioxazin is revealed by comparative heme synthesis assay using embryonic erythroid cells derived from human and rat pluripotent stem cells.
    Article Snippet: Fetal rat anemia from flumioxazin, an N-phenylimide herbicide, is caused by suppression of heme synthesis resulting from inhibition of protoporphyrinogen oxidase (PPO).. A series of studies to investigate the effects of flumioxazin have revealed that developmental toxicity is caused in rats but not in rabbits, and the adverse effects are not likely to occur in humans.. In this study, as a final weight-of-evidence approach for assessing the human safety of flumioxazin, we compared the toxic potential of inhibition of heme synthesis leading to anemia between human and rat embryonic erythroid cells, which were degenerated as the target of flumioxazin in the rat developmental toxicity.

    Article Title: Resveratrol induces apoptosis of bladder cancer cells via miR‑21 regulation of the Akt/Bcl‑2 signaling pathway.
    Article Snippet: Resveratrol and dimethylthiazol-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Human recombinant insulin-like growth factor-1 (IGF-1) was manufactured by R&D Systems (Minneapolis, MN, USA). .. The Annexin V-enhanced green fluorescent protein (EGFP)/propidium iodide (PI) apoptosis detection kit was obtained from KeyGen (Nanjing, China).

    Article Title: In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos.
    Article Snippet: Chimeric pigs harboring organs derived from human stem cells are promising for patient-specific regenerative therapies.. Induced pluripotent stem cells (iPSCs) can contribute to all cell types of the fetus, including germline after injection into embryos.. However, ethical concerns prohibit testing human iPSCs in chimera assays.

    Article Title: Production of extracellular matrix, conditioned media and uses thereof
    Article Snippet: .. Embryoid bodies are formed by treating undifferentiated hES colonies with 1 mg/ml of type IV collagenase (Invitrogen) and resuspending them in a 6-well ultra-low attachment plate (VWR) in the presence of media containing DMEM:F12 (Gibco), 10% knockout serum (Invitrogen), B-27 supplement (Invitrogen), 1 ng/ml mouse noggin (R&D Systems), 1 ng/ml human recombinant Dkk-1 (R&D Systems), and 5 ng/ml human recombinant insulin-like growth factor-1 (IGF-1) (R&D Systems). ..

    Article Title: Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells
    Article Snippet: Peripheral blood mononuclear cells were separated from other blood components using Ficoll-Paque Plus (Merck Millipore, Darmstadt, Germany) and then cells were cultured in Expansion Medium to generate a large population of erythroblasts to be transduced. .. Expansion Medium was composed of Iscove Modified Eagle Medium (EuroClone, Milan, Italy) supplemented with L-Ascorbic Acid (Sigma-Aldrich, Merck Millipore, Darmstadt, Germany; 50 μg/mL), Stem Cell Factor (ISOkine, ORF Genetics, Kopavogur, Iceland; 50 ng/mL), interleukin-3 (R&D Systems, Minneapolis, MN, USA, 10 ng/mL), erythropoietin (R&D Systems; 2U/mL), human recombinant insulin-like growth factor-1 (R&D Systems; 40 ng/mL) and dexamethasone (Sigma-Aldrich, Merck Millipore; 1 μM). ..

    Article Title: Construction and Characterization of EGFP Reporter Plasmid Harboring Putative Human RAX Promoter for in vitro Monitoring of Retinal Progenitor Cells Identity
    Article Snippet: .. So hESCs were 251 transferred to low adherent dishes in order to form EBs in neural induction medium (NIM) 252 containing 1 ng/ml noggin (R&D, 1976-NG), 3 μM IWR (R&D, 5439-DK/CF), and 5 ng/mL 253 human recombinant insulin-like growth factor-1 (IGF-1) (R&D, 291-GI) in DMEM/F12 medium 254 supplemented with 10% KSR, 0.1 mM nonessential amino acids, 2 mM L-glutamine, and 1% B27 255 (Gibco, 17504- 044) for 3 days. .. In the next step, the EBs were dissociated by Accutase (Millipore, 256 SCR005) and were replated on 1 mg/ml laminin and 15 mg/ml Poly-L-ornithine (both from Sigma-257 Aldrich) coated 6-well tissue culture plates.

    In Vitro:

    Article Title: In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos.
    Article Snippet: Chimeric pigs harboring organs derived from human stem cells are promising for patient-specific regenerative therapies.. Induced pluripotent stem cells (iPSCs) can contribute to all cell types of the fetus, including germline after injection into embryos.. However, ethical concerns prohibit testing human iPSCs in chimera assays.

    Activation Assay:

    Article Title: In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos.
    Article Snippet: Chimeric pigs harboring organs derived from human stem cells are promising for patient-specific regenerative therapies.. Induced pluripotent stem cells (iPSCs) can contribute to all cell types of the fetus, including germline after injection into embryos.. However, ethical concerns prohibit testing human iPSCs in chimera assays.

    Incubation:

    Article Title: In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos.
    Article Snippet: Chimeric pigs harboring organs derived from human stem cells are promising for patient-specific regenerative therapies.. Induced pluripotent stem cells (iPSCs) can contribute to all cell types of the fetus, including germline after injection into embryos.. However, ethical concerns prohibit testing human iPSCs in chimera assays.

    Knock-Out:

    Article Title: Production of extracellular matrix, conditioned media and uses thereof
    Article Snippet: .. Embryoid bodies are formed by treating undifferentiated hES colonies with 1 mg/ml of type IV collagenase (Invitrogen) and resuspending them in a 6-well ultra-low attachment plate (VWR) in the presence of media containing DMEM:F12 (Gibco), 10% knockout serum (Invitrogen), B-27 supplement (Invitrogen), 1 ng/ml mouse noggin (R&D Systems), 1 ng/ml human recombinant Dkk-1 (R&D Systems), and 5 ng/ml human recombinant insulin-like growth factor-1 (IGF-1) (R&D Systems). ..

    Modification:

    Article Title: Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells
    Article Snippet: Peripheral blood mononuclear cells were separated from other blood components using Ficoll-Paque Plus (Merck Millipore, Darmstadt, Germany) and then cells were cultured in Expansion Medium to generate a large population of erythroblasts to be transduced. .. Expansion Medium was composed of Iscove Modified Eagle Medium (EuroClone, Milan, Italy) supplemented with L-Ascorbic Acid (Sigma-Aldrich, Merck Millipore, Darmstadt, Germany; 50 μg/mL), Stem Cell Factor (ISOkine, ORF Genetics, Kopavogur, Iceland; 50 ng/mL), interleukin-3 (R&D Systems, Minneapolis, MN, USA, 10 ng/mL), erythropoietin (R&D Systems; 2U/mL), human recombinant insulin-like growth factor-1 (R&D Systems; 40 ng/mL) and dexamethasone (Sigma-Aldrich, Merck Millipore; 1 μM). ..



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